Time-dependent efficacy of combination of silver-containing hydroxyapatite coating and vancomycin on methicillin-resistant Staphylococcus aureus biofilm formation in vitro

Abstract Objective We developed a silver-containing hydroxyapatite (Ag-HA) coating to prevent periprosthetic joint infection (PJI). Methicillin-resistant Staphylococcus aureus (MRSA) is the main PJI-causing bacteria. Previously, we had reported combined effect of Ag-HA and vancomycin (VCM) on MRSA biofilm formation 24 h after inoculation. In this study, investigated time-dependent efficacy VCM Ti discs in vitro by three-dimensional confocal laser scanning microscopic analysis. Results For HA groups, total volumes per area at 96 inoculation were significantly larger than those 48 inoculation, respectively (p < 0.001). contrast, for group, volume was smaller that ( p 0.0001). Moreover, groups Thus, combination might be useful prevention MRSA-associated PJI.